Helmet Shape and Phylogeography of the Treehopper Membracis mexicana.

Membracis mexicana (Hemiptera: Membracidae) is distributed in four biogeographic provinces of Mexico. Field observations indicate that there are different forms of this species, but the distribution of the phenotype and the genetic variation of this species have not been clarified. The aim of this study was to quantify the phenotypic and genetic variation of M. mexicana and determine whether the configuration of biogeographic provinces impacts the distribution of this variation. To achieve this, we analyzed 307 photographs using 19 landmarks and geometric morphometrics to quantify the phenotypic variation in helmets. We sequenced five molecular markers for 205 individuals to describe the phylogeographic pattern. As a result, we identified three morphological configurations of the helmet of M. mexicana and two genetic lineages. The morphotypes are (1) a large and wide helmet with small dorsal spots, (2) a small and narrow helmet with large dorsal spots, and (3) a small and narrow helmet with small spots. Genetic lineages are distributed in southeast and western Mexico. The western lineage corresponds to two helmet morphotypes (1 and 2) and the southeast lineage to morphotype 3. We found that the larger helmets correspond to the western lineage and are distributed in Trans-Mexican Volcanic Belt and Pacific lowlands provinces, whereas the smallest helmets correspond to the southeast lineage and are present in the Veracruzan and Yucatan Peninsula provinces.

Complex evolutionary processes maintain an ancient chromosomal inversion.

Genome re-arrangements such as chromosomal inversions are often involved in adaptation. As such, they experience natural selection, which can erode genetic variation. Thus, whether and how inversions can remain polymorphic for extended periods of time remains debated. Here we combine genomics, experiments, and evolutionary modeling to elucidate the processes maintaining an inversion polymorphism associated with the use of a challenging host plant (Redwood trees) in Timema stick insects. We show that the inversion is maintained by a combination of processes, finding roles for life-history trade-offs, heterozygote advantage, local adaptation to different hosts, and gene flow. We use models to show how such multi-layered regimes of balancing selection and gene flow provide resilience to help buffer populations against the loss of genetic variation, maintaining the potential for future evolution. We further show that the inversion polymorphism has persisted for millions of years and is not a result of recent introgression. We thus find that rather than being a nuisance, the complex interplay of evolutionary processes provides a mechanism for the long-term maintenance of genetic variation.

Prevalencia de marcadores infecciosos en donantes de sangre. Complejo Hospitalario Universitario Ruíz y Páez

Introducción: La infección transmitida por transfusión (ITT) es producto de la inoculación directa de un agente infeccioso específico desde la unidad de sangre al huésped susceptible. Los marcadores serológicos positivos responden a características epidemiológicas no detectadas en miembros de la población aparentemente saludable. Objetivo general: Determinar la prevalencia de marcadores infecciosos y las características sociodemográficas en unidades sanguíneas procesadas de donantes que asisten al banco de sangre del Complejo Hospitalario Universitario "Ruíz y Páez" de Ciudad Bolívar - Estado Bolívar, período junio 2019 - junio 2022. Metodología: Se trató de un estudio de tipo descriptivo, retrospectivo, no experimental. El universo estuvo conformado por 13 016 unidades sanguíneas, 414 muestras fueron reactivas, que reportaron un total de 434 serologías positivas. Resultados: Se apreció una prevalencia global de 3,34 % para cualquier ITT; el marcador infeccioso más frecuente fue sífilis con el 60,83 % y una prevalencia de 2,03 %. Tomando en cuenta las características sociodemográficas predominantes: género masculino, 65,94 %; grupo etario 38-47 años, 28,74 %; ocupación obrera, 24,88 %; provenientes de Ciudad Bolívar, 84,06 %. Las muestras con coinfección representaron un 4,58 %, siendo la combinación VIH+Sífilis la más frecuente 1,45 %. El año 2021 destacó con 35,25 % serologías positivas. Conclusiones: La prevalencia de marcadores infecciosos se considera no despreciable. Se debe reforzar la prevención y cura de las enfermedades infecciosas trasmisibles en miembros de la población aparentemente saludable, así como también redirigir las estrategias en el manejo de seguridad transfusional.

Introduction: Transfusion-transmitted infection (ITT) is the direct inoculation of a specific infectious agent from the blood unit to the susceptible host. Positive serological markers respond to epidemiological characteristics not detected in members of the apparently healthy population. General objective: Determine the prevalence of infection markers and sociodemographic characteristics in processed blood units of donors attending the blood bank of the "Ruíz y Páez" University Hospital Complex in Ciudad Bolívar - Bolivar State, period June 2019 - June 2022. Methodology: This was a descriptive, retrospective, nonexperimental study. The universe consisted of 13 016 blood units, 414 samples were reactive, which reported a total of 434 positive serologies. Results: An overall prevalence of 3.34 % was found for any ITT; the most frequent infection markers were syphilis with 60.83% and a prevalence of 2.03 %. Taking into account the predominant sociodemographic characteristics: male gender, 65.94 %; age group 38-47 years, 28.74 %; labor occupation, 24.88 %; from Ciudad Bolivar, 84.06 %. The samples with coinfection represented 4.58 %, being the combination HIV+Syphilis the most frequent 1.45 %. Year 2021 stood out with 35.25 % positive serologies. Conclusions: The prevalence of infection markers is considered not negligible. The prevention and cure of communicable infectious diseases in members of the apparently healthy population should be strengthened, as well as redirecting strategies in transfusion safety management.

Seroprevalence of viral and bacterial pathogens among malaria patients in an endemic area of southern Venezuela.

BACKGROUND: Malaria remains a leading public health problem worldwide. Co-infections with other pathogens complicate its diagnosis and may modify the disease's clinical course and management. Similarities in malaria clinical presentation with other infections and overlapping endemicity result in underdiagnosis of co-infections and increased mortality. Thus, the aim of this study was to determine the seroprevalence of viral and bacterial pathogens among diagnosed malaria patients in malaria-endemic areas in Venezuela. METHODS: A cross-sectional study was conducted on malaria patients attending three reference medical centres in Ciudad Bolivar, Venezuela. Clinical evaluation and laboratory tests for dengue virus (DENV), chikungunya virus (CHIKV), viral hepatitis [hepatitis A virus (HAV), hepatitis B virus (HBV), and hepatitis C virus (HCV)], and leptospirosis (LEP) were performed by enzyme-linked immunosorbent assays. Previous exposure to these pathogens was defined by the presence of specific immunoglobulin (Ig) G, and co-infection or recent exposure (CoRE) was determined by the presence of specific IgM alone or IgM + IgG. Data analysis considered descriptive statistics. Parameter distribution was statistically evaluated using Kolmogorov-Smirnov test and the necessary comparison tests. Odds ratio (OR) for complications was determined according to CoRE presence with a 95% confidence interval (CI). RESULTS: A total of 161 malaria patients were studied, 66% infected with Plasmodium vivax, 27% with P. falciparum, and 7.5% harboured P. vivax/P. falciparum mixed infection. Previous exposure to DENV (60%) and CHIKV (25%) was frequent. CoRE was confirmed in 55 of the 161 malaria patients (34%) and were more frequent in P. falciparum (49%) than in P. vivax (29%) and mixed malaria patients (25%) (OR = 2.43, 95% CI: 1.39-4.25, P = 0.018). The most frequent CoRE was DENV (15%), followed by HAV (12%), HBV (6.2%), CHIKV (5.5%), and LEP (3.7%); HCV CoRE was absent. Complicated malaria was significantly more frequent in patients with CoRE (56%) than those without CoRE (36%; OR = 2.31, 95% CI: 1.18-4.92, P = 0.013). CONCLUSIONS: We found high CoRE prevalence in malaria patients as determined by serology in the study region; cases were associated with a worse clinical outcome. Further prospective studies with samples from different infection sites and the use of molecular tools are needed to determine the clinical significance of these findings.

Manejo clínico-terapéutico de la malaria grave

La malaria representa un grave problema de salud pública en el país, por su morbilidad y mortalidad. Es importante conocer la patogenia y las manifestaciones clínicas de la malaria grave, en especial revisar el ciclo biológico del parásito, ya que la enfermedad comienza con la ruptura del esquizonte maduro, siendo las primeras manifestaciones clínicas: fiebre y anemia. La infección por Plasmodium falciparum es más severa y es mediada por el fenómeno de secuestro en la microvasculatura venosa profunda, mientras que Plasmodium vivax causa una enfermedad debilitante, rara vez mortal, pero en oportunidades se presentan manifestaciones graves que causan la muerte del paciente. Malaria grave se define por la presencia de signos clínicos y de laboratorio de disfunción de órganos vitales como sistema nervioso central, riñón, gastrointestinal, vías respiratorias y alteraciones hemodinámicas; la cual requiere el rápido reconocimiento de la enfermedad y del grado de severidad. Se debe hacer un manejo de índole general y prestar especial atención a la terapia antimalárica oportuna con Artesunato, primera línea en malaria grave, o Arthemeter o Quinina con Clindamicina según los protocolos nacionales e internacionales, para lograr una evolución satisfactoria. En consecuencia, es un reto enfrentar esta entidad y obliga a la constante actualización en las diferentes opciones cónsonas con las diferentes especies de Plasmodium patógeno.

Malaria represents a serious public health problem in the country, due to its morbidity and mortality. It is of most importance to know the pathogenesis and clinical manifestations of severe malaria, particularly to review the biological cycle of the parasite. The disease begins with the rupture of the mature schizont, with the first clinical manifestations being fever and anemia. Plasmodium falciparum infection is more severe and is mediated by the phenomenon of sequestration in the deep venous microvasculature, while Plasmodium vivax causes a debilitating disease, rarely fatal, but sometimes serious manifestations occur that cause the death of the patient. Severe malaria is defined by the presence of clinical and laboratory signs of dysfunction of vital organs such as the central nervous system, kidney, gastrointestinal, respiratory tract, and pathological hemodynamic changes that requires rapid disease recognition and degree of severity. General management and timely antimalarial therapy with Artesunate, first line in severe malaria, or Arthemeter, or Quinine with Clindamycin following national and international protocols, achieve a favorable outcome. Consequently, it is a challenge to face this entity and requires constant updating in the different options consistent with the different species of pathogenic Plasmodium.

The clinical-epidemiological profile of malaria patients from Southern Venezuela, a critical hotspot in Latin America.

BACKGROUND: Venezuela accounted for 55% of the cases and 73% of the malaria deaths in the Americas in 2019. Bolivar state, in the southeast, contributes > 60% of the country's Plasmodium vivax and Plasmodium falciparum cases every year. This study describes the clinical-epidemiological characteristics of clinical malaria patients in this high-transmission area. METHODS: A prospective study was conducted on patients seeking medical attention in three medical centres in the state capital, Ciudad Bolivar, between June and October 2018. Malaria diagnosis was carried out using microscopy following national standards. Malaria-positive patients were examined for clinical symptoms, and haematological tests were performed at the time of diagnosis. Patients were followed up by telephone to evaluate malaria recurrences. RESULTS: Out of 287 patients, 200 (69.7%) were positive for P. vivax, 69 (24%) for P. falciparum, and 18 (6.3%) had mixed (P. vivax/P. falciparum) infections. Patients' median age was 33 years (IQR 20), 168 (69%) were men, and 40% practiced gold mining as the main occupation. Fever (96.5%), chills (91.3%), and headaches (90.6%) were the most frequent symptoms. At least one symptom associated with severe malaria was observed in 69 out of 161 patients with complete clinical evaluation (42.9%). Plasmodium vivax infections were found in 42 out of 69 (60.9%) severe cases; by contrast, P. falciparum and mixed malaria caused 34.8% (24/69) and 4.4% (3/69) of infections, respectively. Two patients died of cerebral malaria. Mean hemoglobin was lower in the patients infected with P. falciparum than those infected with P. vivax. Regardless of the parasite causing the infection, patients presented high levels of total bilirubin, aminotransferases (AST, ALT), and lactate dehydrogenase (LDH). Out of the 142 patients followed up by phone for three months (49.5% of the 287 patients), 35 (24.7%) reported recurrences. CONCLUSIONS: The high malaria prevalence among young male adults practicing gold mining suggests that this occupation is a significant risk factor. The unexpected high prevalence of P. vivax patients with at least one criteria of severe clinical disease is a matter of concern. Whether it is the result of a lack of timely diagnosis and effective treatment should be explored.

Large-scale mutation in the evolution of a gene complex for cryptic coloration.

The types of mutations affecting adaptation in the wild are only beginning to be understood. In particular, whether structural changes shape adaptation by suppressing recombination or by creating new mutations is unresolved. Here, we show that multiple linked but recombining loci underlie cryptic color morphs of Timema chumash stick insects. In a related species, these loci are found in a region of suppressed recombination, forming a supergene. However, in seven species of Timema, we found that a megabase-size "supermutation" has deleted color loci in green morphs. Moreover, we found that balancing selection likely contributes more to maintaining this mutation than does introgression. Our results show how suppressed recombination and large-scale mutation can help to package gene complexes into discrete units of diversity such as morphs, ecotypes, or species.

Malaria y coinfecciones en una región endémica de Venezuela

Introducción: La malaria continúa siendo un importante problema de salud pública en todo el mundo. Las coinfecciones son un factor de riesgo que incrementa la mortalidad de esta enfermedad. En Venezuela no existen estudios que describan la presencia de coinfecciones en pacientes con malaria. Nosotros determinamos las características clínicas y epidemiológicas de los pacientes con malaria y la presencia de coinfecciones en Ciudad Bolívar, Estado Bolívar, Venezuela. Metodología: Se realizó un estudio descriptivo, correlacional y transversal, que incluyó pacientes diagnosticados con malaria por prueba rápida y/o gota gruesa y extendido de sangre periférica que consultaron en tres centros médicos de Ciudad Bolívar, estado Bolívar, entre junio y noviembre de 2018. Se realizó una evaluación clínica y de laboratorio de cada paciente, las coinfecciones con Dengue (VD), Hepatitis viral (HV) (A, B y C), Leptospirosis (LP), y Chikungunya (VCHIK) fueron evaluadas mediante la técnica de ELISA. Resultados: Un total de 161 pacientes fueron estudiados, 106 (65,8 %) presentaron infección por P. vivax, 43 (26,7 %) por P. falciparum y 12 (7,4 %) tenían malaria mixta (Pf/Pv). La media de edad fue 33,8 (±13,43) años; 103 (63,9 %) fueron hombres, la raza más frecuente fue mestiza (94,4 %); la mayoría de los pacientes (37,3 %) practicaban la minería ilegal. Los síntomas más frecuentes fueron fiebre, escalofríos y cefalea. Anemia leve, trombocitopenia moderada, y compromiso de la función hepática fueron los hallazgos de laboratorio más relevantes en todas las especies parasitarias. Se encontró coinfección en 55/161 (34,2 %) pacientes, siendo más frecuente entre los pacientes con P. falciparum (48,8 %). La coinfección más frecuente fue con VD (14,9 %), seguida de VHA (11,8 %)VHB (6,2 %), VCHIK (5,5 %) y LP (3,7 %). En el grupo de coinfectados fue más frecuente la malaria complicada (56,36 %) que la no complicada (43,63 %) con una diferencia estadísticamente significativa (P=0,018). Conclusión: Se encontró una alta prevalencia de coinfecciones en los pacientes con malaria, y su asociación con la severidad de la Malaria, estos datos epidemiológicos influyen de manera directa en el curso clínico, así como en la mortalidad de la enfermedad. Estos hallazgos deben darse a conocer al personal de salud para la identificación oportuna de coinfecciones en estos pacientes.

Introduction: Malaria continues to be a major public health problem worldwide. Co-infections are a risk factor that increases the mortality of this disease. In Venezuela there are no studies describing the presence of coinfections in patients with malaria. We determine the clinical and epidemiological characteristics of patients with malaria and the presence of coinfections in Ciudad Bolívar, Bolívar state, Venezuela. Methodology: A descriptive, correlational and cross-sectional study was carried out, which included patients diagnosed with malaria by rapid test and / or thick and extended peripheral blood drop that they consulted in three medical centers in Ciudad Bolívar, Bolívar state, between June and November 2018 A clinical and laboratory evaluation of each patient was performed, coinfections with Dengue (DV), viral hepatitis (HV) (A, B and C), Leptospirosis (LP), and Chikungunya (VCHIK) were evaluated using the technique of ELISA Results: A total of 161 patients were studied, 106 (65.8 %) had P. vivax infection, 43 (26.7 %) due to P. falciparum and 12 (7.4 %) had mixed malaria (Pf / Pv ). The mean age was 33.8 (± 13.43) years; 103 (63.9 %) were men, the most frequent race was mestizo (94.4 %); the majority of patients (37.3 %) practiced illegal mining. The most frequent symptoms were fever, chills and headache. Mild anemia, moderate thrombocytopenia, and hepatic function impairment were the most relevant laboratory findings in all parasitic species. Coinfection was found in 55/161 (34.2 %) patients, being more frequent among patients with P. falciparum (48.8 %). The most frequent coinfection was with RV (14.9 %), followed by HAV (11.8 %) HBV (6.2 %), HCV (5.5 %) and LP (3.7 %). Complicated malaria (56.36 %) was more frequent than uncomplicated (43.63 %) with a statistically significant difference (P = 0.018). Conclusion: A high prevalence of coinfections was found in patients with malaria, and its association with the severity of Malaria, these epidemiological data directly influence the clinical course, as well as the mortality of the disease. These findings should be made known to health personnel for the timely identification of coinfections in these patients.

Body dissatisfaction and disordered eating attitudes among adolescents from Portugal and Spain / Insatisfacción corporal y actitudes alimentarias alteradas en adolescentes de Portugal y España

Cross-cultural studies comparing eating attitudes among adolescents from southern-European countries are scarce. We aimed to compare body dissatisfaction, disordered-eating attitudes, awareness and internalisation of the unrealistic-body-ideal, self-esteem, perfectionism and impulsivity among adolescents from Spain and Portugal. Participants were 249 Spanish and 206 Portuguese adolescents (51.2% girls) aged 12-15 (M= 13.3 years, SD= 0.6). A 2 × 2 (country × sex) MANOVA was conducted for each self-reported measure. Girls scored higher on awareness and internalisation of the unrealistic-body-ideal, body-dissatisfaction, disordered-eating attitudes and lower on self-esteem than boys did. Regarding the country, Portuguese adolescents scored higher than Spaniards on eating disorder attitudes, impulsivity and perfectionism. Our findings show significant differences between country and gender, highlighting the importance of specific gender-oriented prevention strategies

Las diferencias transculturales en la insatisfacción corporal y las actitudes alimentarias de riesgo en adolescentes han sido escasamente estudiadas en el sur de Europa. El objetivo de este estudio fue comparar la insatisfacción corporal, las actitudes alimentarias alteradas, la interiorización del ideal estético, la autoestima, el perfeccionismo y la impulsividad de adolescentes de Espana y Portugal. Participaron 249 adolescentes españoles y 206 portugueses (51,2% de chicas) de entre 12 y 15 años (M= 13,3 años; DT= 0,6). Se realizó un MANOVA considerando un diseño 2 × 2 (país × sexo) para cada medida autoinformada. Las chicas obtuvieron puntuaciones más altas en interiorización del ideal estético, insatisfacción corporal, conductas alimentarias alteradas y menor autoestima que los chicos. En cuanto al país, los adolescentes portugueses puntuaron más alto que los españoles en actitudes alimentarias alteradas, impulsividad y perfeccionismo. Nuestros hallazgos muestran diferencias significativas entre país y sexo, destacando la importancia de las estrategias de prevención específicas para cada sexo

Evolution of Trichobaris (Curculionidae) in relation to host plants: Geometric morphometrics, phylogeny and phylogeography.

The family Curculionidae (Coleoptera), the "true" weevils, have diversified tightly linked to the evolution of flowering plants. Here, we aim to assess diversification at a lower taxonomic level. We analyze the evolution of the genus Trichobaris in association with their host plants. Trichobaris comprises eight to thirteen species; their larvae feed inside the fruits of Datura spp. or inside the stem of wild and cultivated species of Solanaceae, such as potato, tobacco and tomato. We ask the following questions: (1) does the rostrum of Trichobaris species evolve according to the plant tissue used to oviposit, i.e., shorter rostrum to dig in stems and longer to dig in fruits? and (2) does Trichobaris diversify mainly in relation to the use of Datura species? For the first question, we estimated the phylogeny of Trichobaris based on four gene sequences (nuclear 18S and 28S rRNA genes and mitochondrial 16S rRNA and COI genes). Then, we carried out morphogeometric analyses of the Trichobaris species using 75 landmarks. For the second question, we calibrated a COI haplotype phylogeny using a constant rate of divergence to infer the diversification time of Trichobaris species, and we traced the host plant species on the haplotype network. We performed an ancestral state reconstruction analysis to infer recent colonization events and conserved associations with host plant species. We found that ancestral species in the Trichobaris phylogeny use the stem of Solanum plants for oviposition and display weak sexual dimorphism of rostrum size, whereas other, more recent species of Trichobaris display sexual dimorphism in rostrum size and use the fruits of Datura species, and a possible reversion to use the stem of Solanaceae was detected in one Trichobaris species. The use of Datura species by Trichobaris species is widely distributed on haplotype networks and restricted to Trichobaris species that originated ca. 5 ±â€¯1.5 Ma. Given that the origin of Trichobaris is estimated to be ca. 6 ±â€¯1.5 Ma, it is likely that Datura has played a role in its diversification.

Pilot assessment of two disordered eating prevention programs. Preliminary findings on maladaptive beliefs related to eating disorders.

AIMS: As eating disorders have severe consequences, they require prevention. We aimed to compare maladaptive beliefs related to eating disorders by following two programs based on media literacy in adolescents at post-test intervention, and after 6 and 12month-follow-ups. The Male and Female Nutrition and Media Literacy Model preventive program (NUT+MEF+MEM+ML in Spanish) and the Theater Alive program are both based on the same contents, the former being presented in a multimedia and interactive format and the latter in a drama format. Both were compared to a control group without intervention, whose participants received usual classes before the assessments. METHOD: Participants were 178 adolescents in the second year of compulsory secondary education from fours schools of Terrassa (Catalonia, Spain). All participants in each school were assigned to the same group, depending on school schedules. A mixed 3 (group: Theater Alive, NUT+MEF+MEM+ML, control)×3 (time: post-test, 6-month-follow-up, 12-month-follow-up) factorial design was used to evaluate the effect on maladaptive beliefs measured using a CE-TCA tool. RESULTS: When compared to the control group, both Theater Alive (d=0.88) and NUT+MEM+MEF+ML (d=0.60) obtained lower scores over time, the latter being not statistically significant. DISCUSSION: The Theater Alive program may produce an effect of cognitive dissonance that might eliminate the discrepancy between the contents of the play and those that are internalized, thus modifying maladaptive beliefs. Participants in the Theater Alive program, as actors in front of an audience, had to defend certain content that was rehearsed over and over again to the point until it became internalized.

Modelo estético, imagen corporal, autoestima y sintomatología de trastornos alimentarios en adolescentes mexicanas y Españolas / Aesthetic model, body image, selfesteem and eating disorders symptomatology in Mexican and Spanish adolescents

El objetivo del trabajo fue analizar la relación del país de origen, la insatisfacción con la imagen corporal, la interiorización del ideal estético y la autoestima en el desarrollo de sintomatología de trastornos de la conducta alimentaria (TCA) en adolescentes de México y España. Participaron 117 mujeres, 68 mexicanas y 49 españolas (Medad= 15,34; DT= 0,51), quienes contestaron el "Cuestionario para la evaluación de los trastornos de la conducta alimentaria" (EDE-Q), el "Cuestionario de imagen corporal" (BSQ), el "Cuestionario de influencias del modelo estético corporal" (CIMEC-26) y la "Escala de autoestima de Rosenberg" (RSES). En cuanto a la sintomatología de TCA, el conjunto de interacciones del país con el resto de variables no mostro influencia significativa (p= 0,721), la insatisfacción con la imagen corporal (p< 0,005) y la interiorización del ideal estético (p= 0,002) mostraron un efecto positivo significativo, mientras que la autoestima y el país un efecto estadísticamente nulo. Estos resultados muestran que no hay diferencias entre la influencia de los factores de riesgo y el desarrollo de sintomatología de TCA en ambos países

The objective of the research was to analyze the relationship between country of origin, body image dissatisfaction, influence of the aesthetic body shape model and self-esteem on the development of eating disorders (ED) symptomatology in Mexican and Spanish adolescents. The participants were 117 female adolescents, 68 from Mexico and 49 from Spain (Mage= 15.34 years old, SD= 0.51), who completed the Eating Disorder Examination Questionnaire (EDEQ), the Body Shape Questionnaire (BSQ), the Aesthetic Body Shape Influence Questionnaire (CIMEC-26, in Spanish) and the Rosenberg Self-Esteem Scale (RSES). Regarding the symptomatology of ED, the interactions of the country with the rest of the variables did not show a significant influence (p= .721), the body image dissatisfaction (p< .005) and the influence of the aesthetic body shape model (p= .002) showed a positive and significant effect, while self-esteem and country of origin did not show any significant effect. According to these results there is no difference between the influence of the risk factors and the development of ED symptomatology in both countries

Phylogeography of specialist weevil Trichobaris soror: a seed predator of Datura stramonium.

Can the genetic structure of a specialist weevil be explained by the geological history of their distribution zone? We analyze the genetic variation of the weevil Trichobaris soror, a specialist seed predator of Datura stramonium, in order to address this question. For the phylogeographic analysis we used the COI gene, and assessed species identity in weevil populations through geometric morphometric approach. In total, we found 53 haplotypes in 413 samples, whose genetic variation supports the formation of three groups: (1) the Transmexican Volcanic Belt (TVB group), (2) the Sierra Madre Sur (SMS group) and (3) the Balsas Basin (BB group). The morphometric analysis suggests that BB group is probably not T. soror. Our results have two implications: first, the phylogeographic pattern of T. soror is explained by both the formation of the geological provinces where it is currently distributed and the coevolution with its host plant, because the TVB and SMS groups could be separated due to the discontinuity of altitude between the geological provinces, but the recent population expansion of TVB group and the high frequency of only one haplotype can be due to specialization to the host plant. Second, we report a new record of a different species of weevil in BB group parasitizing D. stramonium fruits.

Association of biological, psychological and lifestyle risk factors for eating disturbances in adolescents.

We aimed to assess the association of several risk factors for eating disturbances in adolescents. Participants were 448 girls and boys aged 12-15 years. Being female, higher body mass index, internalisation of standard of appearance, perfectionism, body dissatisfaction, number of lifetime addictive behaviours and lower self-esteem were associated with higher eating disturbance scores, whereas frequency of sedentary behaviours and physical activity were not (R(2) ⩾ 41%). Findings suggest the need to guide prevention efforts towards the broad spectrum of individual potentially modifiable factors. A non-specific comprehensive perspective may be adequate to prevent problems related to weight, body image and drug use.

Gender differences found in a qualitative study of a disordered eating prevention programme: What do boys have to say?

Qualitative studies examining gender differences of eating disorder prevention programmes are scarce. We aimed to evaluate gender differences in adolescents who participated in a larger study on effectiveness of a disordered eating prevention programme. Perceptions of eating, female and male aesthetic models, media influences, prevention programmes and emerging topics from 12 school-going boys who received a media-literacy programme (n = 4), media-literacy plus nutrition-awareness programme (n = 4) or neither (n = 4) were explored using Interpretative Phenomenological Analysis and compared with previous results in girls. Findings suggest that the prevention programme is effective for both genders. Gender differences and consumer-culture influences may be considered in future interventions.

Body dissatisfaction and disordered eating among Portuguese and Spanish adolescents: The role of individual characteristics and internalisation of sociocultural ideals.

The aim of this study was to explore the differences in patterns of risk factors for body dissatisfaction and disordered eating attitudes in both female and male adolescents from Portugal and Spain. The sample included 455 adolescents aged 12-16 years (M = 13.28, SD = 0.65) from two urban areas of each country. Body mass index, self-reported self-esteem, perfectionism, internalisation of sociocultural ideals, body dissatisfaction and disordered eating attitudes were assessed. Path analyses provided partial support for a cross-cultural model of body dissatisfaction and disordered eating in Western countries due to the presence of certain differences in the patterns of relationships across sex and country. The findings suggest the importance not only of identifying cultural specificities, even in "neighbouring" countries, but also of developing a global and comprehensive preventive approach that focuses on the influence of the ideal of beauty transmitted by Western societies.

Assessment of two school-based programs to prevent universal eating disorders: media literacy and theatre-based methodology in Spanish adolescent boys and girls.

AIMS: To evaluate the long-term effects of two school-based prevention programs administered to a universal mixed-sex sample of school-going adolescents on disturbed eating attitudes, aesthetic ideal internalization, and other eating disorder risk factors, when compared to a control group. METHODS: Participants were 200 adolescents aged 12-15 selected by means of incidental sampling from second-year compulsory secondary education at schools. An interactive multimedia media literacy program (ML + NUT, Media Literacy and Nutrition) and a program focused on the same topics using dramatic arts (Theatre Alive) were applied and compared with a control group. Pretest, posttest (1 month later), and 5- and 13-month follow-up measurements were taken. Analyses were conducted with two-way mixed 3 × 3 ANCOVA (group × phase) adjusted by baseline levels, body mass index, and sex. RESULTS: Participants in both experimental groups showed significantly higher self-esteem scores than the control group over time. The ML + NUT group also presented lower aesthetic ideal internalization scores than the control group. DISCUSSION: Both programs can benefit students' self-esteem. Moreover, ML + NUT program was useful in reducing thin-ideal internalization. However, differences in body dissatisfaction and disordered eating attitudes were not found. The programs may be protective on the core psychological variables, which are essential to adaptive adolescent development.

Eticidad del pensamiento de Florence Nightingale / Ethics in Florence Nightingale's thought

Introducción: es un gran reto incursionar en la personalidad de Florence Nightingale, mujer que tuvo un papel brillante en su época y que aún hoy está vigente su enfoque sobre enfermería y su aplicación de ciencias como la administración, la estadística y otras, al arte de brindar cuidados. Ella desempeñó múltiples funciones: enfermera, administradora, higienista y docente. Se creció en la Guerra de Crimea y a su regreso creó la primera Escuela de Enfermería. Objetivo: analizar la eticidad del pensamiento de Florence Nightingale. Material y métodos: análisis documental en el estudio de su obra Notas sobre Enfermería. Qué es y Qué no es y bibliografía de otros autores que escribieron sobre ella, su teoría del entorno e informaciones obtenidas por Internet. Sistematización que permitió la organización de la información. Resultados: florence Nightingale añadió a los principios tradicionales de la ética médica los principios de fidelidad y veracidad. Su Juramento refleja sus concepciones y la eticidad y cientificidad de la profesión. Tanto el modelo elaborado como otras contribuciones a la dirección y a la formación de enfermería tienen vigencia actual. Conclusiones: la eticidad de su pensamiento da sentido a los conocimientos, los cuales eran su deseo que formaran parte de la profesión de enfermería. Prevalecen sus actitudes llenas de razón y se aprecian virtudes del carácter e inteligencia. En la actualidad se reconoce un grupo de virtudes, en las que, salvando la distancia en el tiempo, Florence Nightingale se ve reflejada en ellas. Su escuela implica iniciativa, control, responsabilidad, lucha, tratamiento científico de la enfermería y comprensión del método científico(AU)

Introduction: to approach the personality of Florence Nightingale is a challenge. She played an outstanding role in her times and even today her view of Nursing and the application of administration, statistics and other disciplines are still relevant. She was a nurse, a manager, a hygienist and a trainer. She became stronger in the Crimea War, and upon return she founded the first Nursing School. Objective: analyze the ethics in Florence Nightingale's thought. Material and Methods: literature review in the study of her work Notes of Nursing. What is it and what is not and of other papers about her, theory of the environment and also information downloaded from Interne were organized and systematized. Results: florence Nightingale added the principles of fidelity and veracity to the traditional ones of medical ethics. Her pledge expresses the ethics and scientific grounds of Nursing. Both the model she designed and other contributions in management and training are still relevant nowadays. Conclusions: the ethics of her thought makes knowledge meaningful, as she wanted it to be part of nursing. Attitudes that prevail are full of reason and virtues of character and intelligence. Some virtues recognized at present reflect Florence Nightingale _considering the time elapsed. Her school implies initiative, control, responsibility, struggle, a scientific approach to nursing and understanding of the scientific method(AU)

Eticidad del pensamiento de Florence Nightingale / Ethics in Florence Nightingale's thought

Introducción: es un gran reto incursionar en la personalidad de Florence Nightingale, mujer que tuvo un papel brillante en su época y que aún hoy está vigente su enfoque sobre enfermería y su aplicación de ciencias como la administración, la estadística y otras, al arte de brindar cuidados. Ella desempeñó múltiples funciones: enfermera, administradora, higienista y docente. Se creció en la Guerra de Crimea y a su regreso creó la primera Escuela de Enfermería. Objetivo: analizar la eticidad del pensamiento de Florence Nightingale. Material y métodos: análisis documental en el estudio de su obra Notas sobre Enfermería. Qué es y Qué no es y bibliografía de otros autores que escribieron sobre ella, su teoría del entorno e informaciones obtenidas por Internet. Sistematización que permitió la organización de la información. Resultados: florence Nightingale añadió a los principios tradicionales de la ética médica los principios de fidelidad y veracidad. Su Juramento refleja sus concepciones y la eticidad y cientificidad de la profesión. Tanto el modelo elaborado como otras contribuciones a la dirección y a la formación de enfermería tienen vigencia actual. Conclusiones: la eticidad de su pensamiento da sentido a los conocimientos, los cuales eran su deseo que formaran parte de la profesión de enfermería. Prevalecen sus actitudes llenas de razón y se aprecian virtudes del carácter e inteligencia. En la actualidad se reconoce un grupo de virtudes, en las que, salvando la distancia en el tiempo, Florence Nightingale se ve reflejada en ellas. Su escuela implica iniciativa, control, responsabilidad, lucha, tratamiento científico de la enfermería y comprensión del método científico.

Introduction: to approach the personality of Florence Nightingale is a challenge. She played an outstanding role in her times and even today her view of Nursing and the application of administration, statistics and other disciplines are still relevant. She was a nurse, a manager, a hygienist and a trainer. She became stronger in the Crimea War, and upon return she founded the first Nursing School. Objective: analyze the ethics in Florence Nightingale's thought. Material and Methods: literature review in the study of her work Notes of Nursing. What is it and what is not and of other papers about her, theory of the environment and also information downloaded from Interne were organized and systematized. Results: florence Nightingale added the principles of fidelity and veracity to the traditional ones of medical ethics. Her pledge expresses the ethics and scientific grounds of Nursing. Both the model she designed and other contributions in management and training are still relevant nowadays. Conclusions: the ethics of her thought makes knowledge meaningful, as she wanted it to be part of nursing. Attitudes that prevail are full of reason and virtues of character and intelligence. Some virtues recognized at present reflect Florence Nightingale _considering the time elapsed. Her school implies initiative, control, responsibility, struggle, a scientific approach to nursing and understanding of the scientific method.